molecular imager versadoc mp 5000 system (Bio-Rad)
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molecular imager versadoc mp 5000 system
Molecular Imager Versadoc Mp 5000 System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+imager+versadoc+mp+5000+system/molecular+imager+versadoc+mp+4000+system/pmc09806643-53-21-27
Average 90 stars, based on 1 article reviews
Molecular Imager Versadoc Mp 5000 System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/molecular+imager+versadoc+mp+5000+system/molecular+imager+versadoc+mp+4000+system/pmc09806643-53-21-27
Average 90 stars, based on 1 article reviews
molecular imager versadoc mp 5000 system - by Bioz Stars,
2026-09
90/100 stars
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Western Blot:Article Title: Anesthetic Isoflurane Increases Phosphorylated Tau Levels Mediated by Caspase Activation and Aβ Generation Article Snippet: Finally, the antibody to non-targeted protein β-Actin (42 kDa, 1∶5,000, Sigma, St. Louis, MO) was used to control for loading differences in total protein amounts. .. The signal of the Western blot band was detected using the Article Title: Pseudouridylation defect due to DKC1 and NOP10 mutations causes nephrotic syndrome with cataracts, hearing impairment, and enterocolitis Article Snippet: Nylon membranes were blocked with 5% nonfat milk for 1 h, then incubated with an anti-pseudouridine (D-347-3, MBL, diluted [dil.] 1:500 in phosphate-buffered saline [PBS]) primary antibody for 1.5 h, followed by an HRP-conjugated anti-mouse IgG (sc-2005, SCBT; dil. 1:1,000 in PBS) secondary for 1 h, all at room temperature. .. Bands were visualized by chemiluminescence (Western Blotting Luminol Reagent, sc-2048, SCBT) on a SDS Page:Article Title: Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy Article Snippet: To determine protein concentration, HT22 cells were first lysed in a lysis buffer using a BCA kit (Pierce, MA, USA) according to standard protocols. .. Protein samples were severed and separated with 10% SDS-PAGE and transferred onto PVDF membranes where they were blocked with 5% commercial skim milk at room temperature for 1 h and immunoblotted with primary antibodies for NCOA4 (1:500, SANTA CRUZ, sc-373739), Ferritin heavy chain 1 (FTH1) (1:1000, Abcam, ab65080), LC3 (1:1000, CST, #4108), and GAPDH (1:10000, Abcam, ab37168) overnight at 4° C. The following morning, the membranes were washed and incubated with HRP secondary antibodies (1:10000, ASPEN, AS1107) at room temperature for 1 h. Protein concentration was detected using the Incubation:Article Title: Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy Article Snippet: To determine protein concentration, HT22 cells were first lysed in a lysis buffer using a BCA kit (Pierce, MA, USA) according to standard protocols. .. Protein samples were severed and separated with 10% SDS-PAGE and transferred onto PVDF membranes where they were blocked with 5% commercial skim milk at room temperature for 1 h and immunoblotted with primary antibodies for NCOA4 (1:500, SANTA CRUZ, sc-373739), Ferritin heavy chain 1 (FTH1) (1:1000, Abcam, ab65080), LC3 (1:1000, CST, #4108), and GAPDH (1:10000, Abcam, ab37168) overnight at 4° C. The following morning, the membranes were washed and incubated with HRP secondary antibodies (1:10000, ASPEN, AS1107) at room temperature for 1 h. Protein concentration was detected using the Protein Concentration:Article Title: Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy Article Snippet: To determine protein concentration, HT22 cells were first lysed in a lysis buffer using a BCA kit (Pierce, MA, USA) according to standard protocols. .. Protein samples were severed and separated with 10% SDS-PAGE and transferred onto PVDF membranes where they were blocked with 5% commercial skim milk at room temperature for 1 h and immunoblotted with primary antibodies for NCOA4 (1:500, SANTA CRUZ, sc-373739), Ferritin heavy chain 1 (FTH1) (1:1000, Abcam, ab65080), LC3 (1:1000, CST, #4108), and GAPDH (1:10000, Abcam, ab37168) overnight at 4° C. The following morning, the membranes were washed and incubated with HRP secondary antibodies (1:10000, ASPEN, AS1107) at room temperature for 1 h. Protein concentration was detected using the Software:Article Title: Hydrogen sulfide alleviates hypothyroidism-induced myocardial fibrosis in rats through stimulating autophagy and inhibiting TGF-β1/Smad2 pathway Article Snippet: .. Finally, the bands were visualized by adding with enhanced chemiluminescence complied with the manufacturer’s instruction (Beyotime) and detected by using a Imaging:Article Title: Acupuncture at PC6 prevents cardiac hypertrophy in isoproterenol-treated mice. Article Snippet: .. Subsequently, immunoreactive proteins were detected using a |